It is possible to get transfer efficiencies of 60% to 80% for proteins between 14 and 116 kDa. The Trans-Blot Turbo system is a rapid protein transfer apparatus that can transfer protein to membrane in as little as 3 minutes. Semi-dry blotters are designed to quickly and efficiently transfer proteins and nucleic acids from gels onto blotting membranes. Your subscription information already exists, we will send you an email with specific instructions to manage your existing subscription profile. Instructional video to guide you through the features, benefits, and operation of the Trans-Blot Turbo system. $(document).ready(function(){$('.helpemail').remove();$('.countrylist td a:contains("Brazil")').text('Brasil');$('.countrylist td a:contains("Mexico")').text('México');$('.countrylist td a:contains("Austria")').text('Österreich');$('.countrylist td a:contains("Belgium")').text('België/Belgique/Belgien');$('.countrylist td a:contains("Denmark")').text('Danmark');$('.countrylist td a:contains("Finland")').text('Suomi');$('.countrylist td a:contains("Germany")').text('Deutschland');$('.countrylist td a:contains("Italy")').text('Italia');$('.countrylist td a:contains("Norway")').text('Norge');$('.countrylist td a:contains("Russian Federation")').text('Россия');$('.countrylist td a:contains("Spain")').text('España');$('.countrylist td a:contains("Sweden")').text('Sverige');$('.countrylist td a:contains("Switzerland")').text('Schweiz/Suisse');$('.countrylist td a:contains("The Netherlands")').text('Nederland');$('.countrylist td a:contains("Japan")').text('日本');$('.countrylist td a:contains("China")').text('中国');$('.countrylist td a:contains("Korea")').text('한국');});Please select your location to view the products, information, and services available, including news, promotions and events.. Rat liver extract was focused on Bio-Rad's ReadyStrip™ 11 cm pH 5–8 IPG strips and separated on AnykD™ Criterion™ TGX™ gels. In order to ensure quality, the entire range is rigorously tested on various parameters by our quality … Purchase chemiluminescent western blot detection reagents at a 25% discount and get a free sample of EveryBlot Blocking Buffer. The semi-dry transfer has several advantages over a traditional wet transfer including the ease of setup, minimal solvent required, and higher throughput. To receive the latest news, promotions, and more, sign up for Bio-Rad updates by entering your email address below. Please reenter your email address in the correct format. There are no questions associated with this feedback form. $(document).ready(function() { var region = $("#headlangID .lang strong").html(); $(".footer-nav > a:nth-child(3)").after(" | Web Accessibility");});Trademarks | The polyacrylamide gels must be equilibrated in transfer … There are no assessment buttons associated with this feedback form. High throughput — 4 mini gels or 2 midi gels per run. Traditional semi-dry transfer system transfers proteins ranging from 10–100 kD in 15–60 minutes. A semi-dry transfer apparatus is one of the easiest ways to transfer proteins to nitrocellulose prior to a western blot. The nitrocellulose membranes were subsequently stained with SYPRO Ruby and imaged on a VersaDoc™ MP 4000 system. Compared to traditional tank blotters, these devices carry out electrophoretic transfer using significantly less transfer … Semi-Dry Electroblotting. Better Transfer of Different Types of Proteins, Hydrophobic Interaction Chromatography Resins, Protein Expression and Purification Series, pGLO Bacterial Transformation and GFP Kits, Trans-Blot Turbo System Instructional Video, Trans-Blot SD Semi-Dry Blotting Apparatus, Protein Electrophoresis and Western Blotting, Bio-Rad Awards Scholarships to High School and Undergraduate Science Teachers to Attend Virtual National Science Teaching Association Teaching Conference, Bio-Rad Introduces Reliance Select cDNA Synthesis Kit, a Reverse Transcription Solution that Efficiently Converts RNA to DNA, Cell Therapy Analytical Development Summit, American Society of Hematology Annual Meeting. The semi-dry transfer has several advantages over a traditional wet transfer including the ease of setup, minimal solvent required, and higher throughput. We are an acclaimed name engaged in offering our precious clients a superior quality range of Semi Dry Blotting Apparatus that is mainly used in bio labs and hospitals. Turbo Transfers with Trans-Blot® Turbo™ Transfer Packs. A constant current (0.1 to 0.4 A) or voltage (10 to 24 V) is applied for 10 minutes to one hour. Trans-Blot ® SD Semi-Dry Transfer Cell. WSE-4025 HorizeBLOT 2M Rapid Semi-Dry Blotter. Standards in lane 1 are Precision Plus Protein™ Unstained standards, with a top band of 250 kD MW.